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. 2018 Jul 5;8:10188. doi: 10.1038/s41598-018-28372-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design: Timing of the neonatal treatment and of the injections, expressed in weeks, and the experimental procedures performed with two different and independent batches of mice. After corticosterone administration during the neonatal phase (postnatal days, PND, 1–10), mice received 5 injections of GAS homogenate or Phosphate Buffer Saline (PBS), formulated with the indicated adjuvants (CFA = Complete Freund’s adjuvant; IFA = Incomplete Freund’s adjuvant). At sacrifice, we collected: in Batch I, plasma samples for antibody and cytokine determination and brain samples for immunohistochemistry; in Batch II, we collected brain areas for monoamine determinations and RNA sequencing.