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. 2018 Jul 5;8:10188. doi: 10.1038/s41598-018-28372-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Hair corticosterone (CORT) concentrations measured at weaning in control (WATER) and CORT-treated subjects (n = 11 per group); (b) Plasma CORT concentrations measured at sacrifice (i.e. two weeks after the fifth GAS injection; n = 8–12 per group): log-transformed data were used for the statistical analysis; actual concentration data are reported for the sake of clarity. CORT-treated subjects showed increased concentrations of CORT both in the short- (weaning) and long-term (adulthood). ^*p < 0,05 compared to respective WATER controls; (c) Western Blot analysis of GAS extracts probed with pooled sera from mice treated with GAS homogenates or adjuvant alone as Control, neonatal CORT and GAS homogenates or neonatal CORT and adjuvant. Lane 1, Western Blot results after probing with sera of control mice injected with four doses of adjuvant alone; lane 2, Western Blot results after probing with sera of mice injected with four doses of GAS homogenates; lane 3, Western Blot results after probing with sera of mice injected with four doses of neonatal CORT and adjuvant; lane 4, Western Blot results after probing with sera of mice injected with four doses of neonatal CORT and GAS homogenates. Lanes have been cropped from different parts of the same gel (see Supplementary Fig. 2).