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. 2018 Jul 5;8:10156. doi: 10.1038/s41598-018-28525-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pictures of Pseudomonas species grown on NB agar supplemented with calcium and 0.1% (w/v) BB over the course of two days. Pictures are of the same colony on day one and day two. The relative pH of the media can be determined by the change in the color of BB. Pictures of the color change in NB agar supplemented with calcium and 0.1% (w/v) BB from a pH of 6.4 to 8.0 is shown below the pictures of the bacterial colonies. This assay was repeated three separate times and the pictures are representative of those assays.