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. 2018 Jul 5;8:10156. doi: 10.1038/s41598-018-28525-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEM images at 18,000x magnification of the agar adjacent to bacterial colonies for P. putida KT2440, P. syringae pv. morsprunorum 5795, P. syringae pv. tomato DC3000, and P. fluorescens Pf0-1 after 12 hours and six days of growth. 2 µm scale bars are indicated in the lower right-hand corner of the images. Arrows labelled “CM” points to calcium phosphate that has formed on the agar. Imaging of the agar adjacent to colonies was performed three times using three separate biological replicates. This image is representative of what was observed with the other replicates.