Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 5;8:10180. doi: 10.1038/s41598-018-28242-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Inhibition of transcytosis of infectious HIV-1 by human anti-HIV-1 env IgM monoclonal antibodies. The in vitro neutralizing Human anti-HIV-1 IgM MO101, MO97, and MO99 and the control non-neutralizing MO6 with or without 5 µg/mL rgp160 were added together with HIV-1 SI 6794 infected PBMC during 24 h on the apical side to assess inhibition of HIV-1 transmission/transcytosis from the apical side to the basolateral side across the human epithelial cell line Caco-2-pIgR+. The inhibition of HIV transcytosis was assessed intracellularly in the cells and in the basolateral supernatant by p24 ELISA. (A) The in vitro neutralizing Human anti-HIV-1 IgM MO101 and the control non-neutralizing MO6 with or without rgp160 (p < 0.01), (B) the in vitro neutralizing anti-HIV-1 IgM MO99 (p < 0.01) and MO97 (p = 0.05) with or without rgp160. Mann Whitney U test was used to determine if the samples were significantly different than the negative control (**p < 0.01.and *p < 0.05). The bars represent the median percent and range inhibited HIV-1 p24 antigen in vitro from two to three repeated experiments. Comparisons of inhibited HIV-1 p24 were performed between control IgM (MO6) and the anti-HIV-1 neutralizing IgM MoAbs.