Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 5;8:10151. doi: 10.1038/s41598-018-28559-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of methionine deficiency on key factors of the mTOR and GCN2/eIF2α signaling pathways Phosphorylation of (A), ribosomal protein S6 kinase 1 (RPS6K1) (B) eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) and (C) eukaryotic translation initiation factor 2α (eIF2α) proteins in liver of trout fed the control diet (CTL) or the methionine deficient diet (MD) and sampled 2 h after the last meal. Western blot analysis was carried out on six individual samples per treatment, and a representative blot is shown (Source data for each blot are available in Supplementary Fig. 1). Graphs show the ratio of the amount of the phosphorylated protein: the total amount of the targeted protein. The mRNA levels of (D) asparagine synthetase (asns) and (E) DNA-damage inducible transcript 3 (ddit3) were measured using quantitative real-time RT-qPCR assays in liver 2 and 16 h after the last meal. Expression values are normalized with the eukaryotic translation elongation factor 1 α 1 (eef1a1) mRNA. Values are means (n = 6), with standard error of the mean represented by vertical bars. *was used to indicate significant difference between treatment among the two dietary group (P < 0.05; one-way ANOVA followed by the student-Newman-Keuls multiple-comparison test).