Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 5;8:10151. doi: 10.1038/s41598-018-28559-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Methionine deficiency decreases the oxidative status. (A) Aconitase activity, (B) quantity of total glutathione (total GSH), reduced glutathione (GSH) and oxidized glutathione (GSSG), (C) ratio GSH/GSSG, (D) levels of protein carbonyls in liver of trout fed control diet (CTL) or deficient diet (MD) and sampled 16 h after the last meal. Levels of protein carbonyls were measured by Oxyblot. DNP-derivatized liver tissue lysates were analysed by Western blot for the presence of oxidized protein. Western blot analysis was carried out on six individual samples per treatment, and a representative blot is shown (Source data are available in Supplementary Fig. 2). Graphs show the ratio of the amount of the oxidized protein: β-tubulin (TUBB) used as a loading control. Values are means (n = 6), with standard error of the mean represented by vertical bars. *was used to indicate significant difference between treatment among the two dietary group (P < 0.05; t-test).