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. 2018 Jul 5;8:10151. doi: 10.1038/s41598-018-28559-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methionine deficiency has no impact on antioxidant defence The mRNA levels of (A) superoxide dismutase 1 (sod1), (B) superoxide dismutase 2 (sod2), (C) glutathione-S-transferase π (gstπ) and (D) glutathione-disulfide reductase (gsr) were measured using quantitative real-time RT-qPCR assays in liver of trout fed control (CTL) or methionine deficient diet (MD) and sampled 16 h after the last meal. Expression values were normalized with the eukaryotic translation elongation factor 1 α 1 (eef1a1) mRNA. Catalase activity (E) was measured by oxygraphe 16 h after the last meal. Values are means (n = 6), with standard error of the mean represented by vertical bars. *was used to indicate significant difference between treatment among the two dietary group (P < 0.05; t-test).