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. 2018 Jul 5;8:10151. doi: 10.1038/s41598-018-28559-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methionine deficiency affects oxidative phosphorylation and induces energetic stress (A). Activities of Complex I, Complex II and Complex IV of OXPHOS system in the liver of trout fed the control diet (CTL) or the methionine deficient diet (MD) and sampled 16 h after the last meal. Analysis was carried out on six individual samples per treatment. (B) Phosphorylation of AMPK in the liver of trout fed the control diet (CTL) or the methionine deficient diet (MD) and sampled 16 h after the last meal. Western blot analysis was carried out on six individual samples per treatment, and a representative blot is shown (Source data are available in Supplementary Fig. 3). Graph show the ratio of the amount of the phosphorylated protein: the total amount of the targeted protein. Values are means (n = 6), with standard error of the mean represented by vertical bars. *was used to indicate significant difference between treatment among the two dietary group (P < 0.05; t-test).