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. 2018 Jul 5;8:10151. doi: 10.1038/s41598-018-28559-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methionine deficiency leads to induction of the PINK11/PARKIN axis. Western blot analysis of (A) PARKIN, (B) Ubiquitin-p^Ser65 and (C) Mfn2 in the liver of trout fed the control diet (CTL) or methionine-deficient diet (MD) and sampled 16 h after feeding the last meal. Western blot analysis was carried out on six individual samples per treatment, and a representative blot is shown (Source data for each blot are available in Supplementary Figs 6 and 7). Graphs show the ratio of the targeted protein: β-tubulin (TUBB) or the total amount of the targeted protein used as a loading control. Values are means (n = 6), with standard error of the mean represented by vertical bars. *was used to indicate significant difference between treatment among the two dietary group (P < 0.05; t-test).