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. 2018 Jul 5;8:10187. doi: 10.1038/s41598-018-28473-z

Table 1.

Summary of hybrid genome assemblies of two marine fungi and statistical summary of reads from Roche 454 and Illumina.

Calcarisporium sp. KF0525 Pestalotiopsis sp. KF079
Genome size 36.8 Mb 47.5 Mb
Scaffolds 2274 318
N25* (#scaffolds) 162.8 kb (43) 821.7 kb (11)
N50* (#scaffolds) 95.7 kb (115) 429.3 kb (32)
N75* (#scaffolds) 51.3 kb (263) 249.3 kb (67)
GC content (%) 50.6 52.1
No. of Genes 15,459 22,626
Read Statistics
Roche 454 No of reads 773,371 824,828
Total length 441,946,919 424,413,772
Average read length 571.5 514.5
Genomic coverage 9.3× 11.5×
Illumina No of reads 77,557,838 79, 803, 010
Total length 7, 833,341,638 8, 060,104,010
Average read length 101 101
Genomic coverage 169× 219×

*Length of the scaffold until which sum of lengths of scaffolds are reached to 25%, 50% and 75% of total assembled genome size are called N25, N50 and N75 respectively.

#Scaffolds – Number of scaffolds in the assembled genome that constitute particular N25 or N50 or N75.