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. 2018 Jul 5;8:10196. doi: 10.1038/s41598-018-28542-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell cytotoxicity assay and proliferation assay. (A) CCK-8 assay of cell viability for SK-BR-3 cells incubated with DNS at various concentration. (B) CCK-8 assay of cell viability for SK-BR-3 cells incubated with AptDzy-DNS at different hours. (C) RT-PCR characterization of HER2 mRNA expression for SK-BR-3 cells incubated without and with AptDzy-DNS. The data error bars indicate means ± SD (n = 5). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test), (D) Western blot characterization of HER2 expression for SK-BR-3 cells incubated with 0.25 (Lane 1), 0 (Lane 2), 0.5 (Lane 3), 0.25 (Lane 4), and 0.125 (Lane 5) μM AptDzy-DNS.