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. 2018 Jul 5;8:10223. doi: 10.1038/s41598-018-28217-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The chromosome target site bias of SB and TcB. The transposon insertion sites of TcB (A) and SB (B) are plotted relative to ORF positions. Each ORF is divided into 25 equal-sized segments and the number of insertions in each segment is displayed. Insertion sites in intergenic regions closer to the 5′ or 3′ end of an ORF are plotted upstream or downstream of the ORFs, respectively. X-axis means distance to ORF while y-axis represents integration events in vivo. (C) WebLogos generated from insertion sites of TcB and SB transposons using the website at www.weblogo.berkeley.edu. (D) The Venn diagrams showing the number of duplicated insertion sites by two TcB libraries, two SB libraries, as well as one TcB library and one SB library.