Skip to main content
. 2018 Jun 29;9:344. doi: 10.3389/fendo.2018.00344

Figure 5.

Figure 5

XRK3F2 rescues H3K9ac levels and subsequent osteogenic differentiation of MM hBMSCs. Human BMSC samples were expanded in proliferation media and harvested for ChIP-qPCR at either: (Day 0) the day of the switch into osteogenic media +/– XRK3F4 (5 μM) or (Day 4) after four days of differentiation. In the control healthy donor (HD)-BMSC, the d0+XRK3F2 sample was kept in proliferation media from d0 through d4 in the presence of XRK3F2. Anti-H3K9Ac ChIP-qPCR analysis of (A) HD-hBMSC (n = 2) and (B) MM-hBMSC (MM) (n = 4, MM patient samples MM1-4, Table 3) using amplicons +185 and +66065 relative to the hRunx2 P1 TSS. Day 0 (d0) anti-H3K9Ac ChIP amplicon +185 N sample result was used as the reference sample for other samples on graphs and ΔΔCt shown. (C) Healthy donor BMSC were co-cultured with MM1.S for 72 h in proliferation media, MM cells were removed and the remaining hBMSC were subjected to osteogenic differentiation for 5 days +/– XRK3F2 (5 μM). qPCR profiles for Runx2 (C) and Gfi1 (D) mRNA are shown. SEM for 3 experimental wells and representative of 2 biological replicates is indicated. *p ≤ 0.05; **p ≤ 0.01.