Table 2.
Murine and human ChIP-qPCR Runx2-P1 primers.
| ChIP amplicons* | Forward (5′->3′) | Reverse (5′->3′) |
|---|---|---|
| Murine −670 | AAGGCAAACAGAAGGAAGCA | TGCTGCTTTGCAGTAATTCG |
| Murine −36 (3) | TGAGGTCACAAACCACATGA | TGAAGCATTCACACAATCCAA |
| Murine +150 (5) | CGTTTTGTTTTGTTTCCTTGC | CCCAGTCCCTGTTTTAGTTG |
| Murine +363 (6) | CAGGGACTGGGTATGGTTTG | ACGCCATAGTCCCTCCTTTT |
| Murine +33130 | AGGTAGCCCAGCAAAAACCT | CCCCTCTGTGAGCCAAAATA |
| Human +185 | CACCGAGACCAACAGAGTCA | TGGTAACATGTGAAAAGCAAAGA |
| Human +66065 | AAGGCCCCACCTCTAACACT | AGACAACAGGCGAGGCTAAA |
*
Numbers represent midpoints of amplicons relative to the Runx2-P1 transcription start site. Numbers in parentheses were used to designate the amplicons in our previous publication (31).