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. 2018 Jun 29;9:1503. doi: 10.3389/fimmu.2018.01503

Figure 1.

Figure 1

Serum amyloid A (SAA)3 expression in dextran sulfate sodium (DSS)-induced mouse colitis model. (A) Relative mRNA expression levels of Saa1 and Saa3 in colonic tissue of wild-type (WT) mice treated with control (water only) or DSS (3.5% in water), based on quantitative RT-PCR [mean ± standard margin of error (SEM), n = 5, triplicate]. (B) Relative expression levels of SAA3 protein in colonic tissue of WT mice without (open bar) and with (closed bar) 3.5% DSS treatment, based on Western blot analysis (mean ± SEM, n = 5, triplicate). (C) Representative immunofluorescence images showing SAA3 protein expression (red) in mouse colonic epithelium (green) before (day 0) and 3 or 7 days after giving 3.5% DSS in drinking water (n = 5). Scale bar, 20 µm. **p < 0.01 between groups. Male and female mice 8–10 weeks of age were used in all experiments.