Skip to main content
. 2018 Jun 29;9:1503. doi: 10.3389/fimmu.2018.01503

Figure 2.

Figure 2

Dextran sulfate sodium (DSS)-induced pathological changes in wild type (WT) and Saa3−/− mice. Male and female mice 8–10 weeks of age were used in the experiments. (A) Body weight change in WT (black) and Saa3−/− (red) mice (n = 10) receiving 3.5% DSS in drinking water for up to 7 days. (B) Mice were sacrificed on day 7 and colons were excised. Macroscopic examination of colon lengths in WT and Saa3−/− mice (left) and statistical analysis of colon lengths (right, n = 5). (C) Representative images of hematoxylin and eosin stained colon sections from control and DSS-treated mice after 7 days. Scale bars, 50 and 20 micrometers in upper and lower panels, respectively. The histological scores of colon pathology are shown in the right panel (n = 5). (D) Survival rates of mice treated with 3.5% DSS for 7 days followed by normal drinking water for another 8 days (n = 10 each). (E) Representative images showing proliferative cells (Ki67+) in colon of mice with DSS-induced colitis. Quantification of Ki67+ cells in each crypt is shown in right panel (n = 5). Scale bars, 20 micrometers. All quantitative data are mean ± standard margin of error based on triplicate measurements. *p < 0.05 and **p < 0.01 between WT and Saa3−/− mice.