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. 2018 Jun 29;9:1503. doi: 10.3389/fimmu.2018.01503

Figure 4.

Figure 4

Amelioration of dextran sulfate sodium (DSS)-induced colitis by recombinant SAA3 (rSAA3). (A) Weight loss was monitored every day in Saa3−/− mice receiving DSS with rSAA3 (blue) or without rSAA3 (red) or drinking water only (black). (B) A representative image (left) and quantitative analysis of colon length measurement (right, n = 5). (C) Representative images of paraffin sections of distal colon tissue were stained with hematoxylin and eosin (left panel), and pathology scores were obtained (right panel, n = 5). Scale bars, 20 micrometers. (D) Relative mRNA levels of Reg3β (left) and Reg3γ (right) in Saa3−/− colitis mice receiving water or DSS with or without rSAA3 were quantitatively analyzed (n = 5). (E) Colonic tissue explants (n = 5 each, receiving water, DSS, and DSS + rSAA3) were collected 7 days after treatment and then cultured ex vivo for 24 h for measurement of secreted IL-22 by ELISA. Male and female mice 8–10 weeks of age were used in the above experiments. For all quantitative data above, *p < 0.05 and **p < 0.01 between different groups.