Figure 8.

Adoptive transfer of serum amyloid A (SAA)3-treated neutrophils ameliorates dextran sulfate sodium (DSS)-induced colitis. (A) Bone marrow neutrophils from wild-type (WT) mice were incubated for 6 h with or without 1 µM of recombinant SAA3 (rSAA3) and 5 × 10⁵ cells were intravenously injected into a Saa3^−/− mouse 1 day after DSS administration. (B) IL-22 released into the culture medium was measured by ELISA. (C) Body weight loss was monitored every day in Saa3^−/− mice receiving rSAA3-treated neutrophils (blue) or vehicle-treated neutrophils (red). Untreated controls were included. (D) The lengths of colons were measured with a representative image shown on the left (n = 5 each condition). (E) Representative images of hematoxylin and eosin stained paraffin sections of distal colon tissue (left panels) and pathology scores (right; n = 5). Scale bars, 20 µm. (F) Relative mRNA levels of the antimicrobial peptides Reg3β (left) and Reg3γ (right) in colon tissue samples. Male and female mice 8–10 weeks of age were used in these experiments. All data were collected with triplicate measurements and shown as mean ± standard margin of error. *p < 0.05 and **p < 0.01.