Table 3.
Identity and function of a selection of differentially expressed protein-encoding gene families between the HUS and non-HUS group.
| Gene family | Log2 FC | Gene Ontology | Annotation | ID | Putative function | Present in no. strains | |
|---|---|---|---|---|---|---|---|
| HUS | non-HUS | ||||||
| 4021 | 1.56 | Nucleic acid phosphodiester bond hydrolysis | Secreted protein Hcp | hcp | Type VI secretion system | 15 | 12 |
| 3905 | 2.37 | NA | IcmF-related protein | IcmF | Type VI secretion system | 15 | 12 |
| 3990 | 2.05 | NA | Type VI secretion protein VasI | – | Type VI secretion system | 15 | 12 |
| 3907 | 2.37 | Proteolysis | ClpB protein | clp | Type VI secretion system | 15 | 12 |
| 58 | 2.99 | NA | VgrG protein | vgrG | Type VI secretion system | 15 | 12 |
| 99 | 3.00 | Cellular response to phosphate starvation, dephosphorylation, cellular response to anoxia | core protein | – | Rhs protein | 15 | 12 |
| 23 | 3.04 | Transposition, DNA-mediated | Mobile element protein | – | – | 15 | 12 |
| 3790 | −2.97 | Pathogenesis | Probable glutamate/gamma-aminobutyrate antiporter | – | – | 13 | 15 |
| 3860 | 1.31 | Cell adhesion | type 1 fimbrae adaptor subunit FimF | ydeS | F9 fimbriae | 15 | 13 |
| 215 | 1.39 | Oxidation-reduction process | Mobile element protein | Z4324 | OI-122 | 15 | 14 |
| 611 | 1.19 | Oxidation-reduction process | Enterotoxin | espL | OI-122 | 15 | 14 |
| 4065 | 2.48 | NA | FIG00638171: hypothetical protein | EDL933_4193 | OI-122 | 15 | 12 |
| 3569 | 1.25 | NA | FIG00638856: hypothetical protein | nleB | OI-122 | 15 | 14 |
| 2325 | 1.08 | Oxidation-reduction process | Unknown function | nleE | OI-122 | 15 | 14 |
| 3869 | −1.63 | Oxidation-reduction process, response to stress | Type III secretion chaperone protein for YopD (SycD) | – | – | 13 | 15 |
| 3865 | 1.32 | NA | PilT | – | – | 15 | 13 |
| 4042 | 1.76 | NA | FIG01200701: hypothetical protein | ygiC | Yqi-like fimbriae | 15 | 12 |
| 4014 | 2.15 | Cell adhesion | Uncharacterized fimbrial-like protein ygiL precursor | YgiL | Yqi-like fimbriae | 15 | 12 |
| 3912 | 2.36 | Transport | Uncharacterized outer membrane usher protein yqiG precursor | ygiG | Yqi-like fimbriae | 15 | 12 |
| 3908 | 2.98 | NA | Alpha-fimbriae usher protein | – | Cs1-like fimbriae | 15 | 12 |
| 4017 | 1.94 | NA | Alpha-fimbriae major subunit | – | Cs1-like fimbriae | 15 | 12 |
| 4010 | 1.99 | Cell adhesion | Putative fimbrial-like protein | yraH | Yra fimbriae | 15 | 12 |
| 3997 | 1.93 | Pilus organization | Chaperone protein fimC precursor | yraI | Yra fimbriae | 15 | 12 |
| 3911 | 2.45 | Transport | type 1 fimbriae anchoring protein FimD | yraJ | Yra fimbriae | 15 | 12 |
| 2526 | −1.16 | Oxidation-reduction process, response to oxidative stress, negative regulation of transcription, DNA-templated | Flavoprotein wrbA | – | Insertion site of Stx phage | 15 | 15 |
| 62 | 1.05 | NA | putative virulence protein | insQ | IS609 transposase B | 15 | 14 |
| 90 | 2.08 | Transposition, DNA-mediated | Mobile element protein | insP | IS609 transposase A | 15 | 13 |
| 2890 | −1.14 | Cell adhesion | Universal stress protein F | uspF | Insertion site of phage | 15 | 15 |
| 3840 | 1.18 | NA | HTH-type transcriptional regulator mlrA | mlrA | Insertion site of Stx phage | 15 | 13 |
| 4012 | 1.90 | Cell adhesion | Major fimbrial subunit StfA | YfcV | Yfc fimbriae | 15 | 12 |
| 3849 | 1.55 | Regulation of transcription, DNA-templated, transcription, DNA-templated, DNA integration, DNA recombination | type 1 fimbriae regulatory protein FimE | fimE | type-1-fimbriae | 15 | 13 |
| 3856 | 2.29 | Cell adhesion | type 1 fimbriae major subunit FimA | fimA | type-1-fimbriae | 15 | 13 |
| 3858 | 1.68 | Cell adhesion | type 1 fimbriae protein FimI2C unknown function | fimI | type-1-fimbriae | 15 | 13 |
| 3841 | 1.72 | Pilus organization | chaperone FimC | fimC | type-1-fimbriae | 15 | 13 |
| 1 | 1.13 | NA | Mobile element protein | – | type-1-fimbriae | 15 | 14 |
| 3859 | 1.62 | Cell adhesion | type 1 fimbrae adaptor subunit FimF | fimF | type-1-fimbriae | 15 | 13 |
| 3863 | 1.40 | Cell adhesion, pilus organization | type 1 fimbrae adaptor subunit FimG | fimG | type-1-fimbriae | 15 | 13 |
| 3822 | 1.67 | Cell adhesion | mannose-specific adhesin FimH | fimH | type-1-fimbriae | 15 | 13 |
| 4106 | −2.51 | Cell adhesion | FIG01069793: hypothetical protein | – | – | 11 | 15 |
| 3624 | −1.04 | Pathogenesis | Methionine aminopeptidase (EC 3.4.11.18) | – | – | 14 | 15 |
| 4040 | 2.12 | Nucleic acid phosphodiester bond hydrolysis | CRISPR-associated protein2C Cas2 | cas2 | CRISPR/Cas | 15 | 12 |
| 3964 | 2.24 | nucleic acid phosphodiester bond hydrolysis, defense response to virus, maintenance of CRISPR repeat elements | CRISPR-associated protein Cas1 | cas1 | CRISPR/Cas | 15 | 12 |
| 4003 | 2.18 | NA | CRISPR-associated protein2C Cse3 family | cas6 | CRISPR/Cas | 15 | 12 |
| 3989 | 1.94 | Defense response to virus | CRISPR-associated protein2C CT1976 | hyp | CRISPR/Cas | 15 | 12 |
| 3950 | 2.09 | NA | CRISPR-associated protein2C Cse4 family | cse4 | CRISPR/Cas | 15 | 12 |
| 4172 | 3.31 | Cell adhesion | Fimbriae-like adhesin SfmA | – | LPFB1 fimbriae | 15 | 10 |
| 4168 | 3.45 | Pilus organization | Putative fimbrial chaperone protein | – | LPFB1 fimbriae | 15 | 10 |
| 4158 | 4.21 | Transport | type 1 fimbriae anchoring protein FimD | – | LPFB1 fimbriae | 15 | 10 |
| 4163 | 3.59 | Cell adhesion | Putative fimbrial protein | – | LPFB1 fimbriae | 15 | 10 |
| 3639 | −1.07 | Pathogenesis | Phosphate transport system regulatory protein PhoU | – | – | 14 | 15 |
| 3451 | 1.52 | Pathogenesis | FIG00638466: hypothetical protein | – | – | 15 | 14 |
| 3588 | 1.06 | Chemotaxis, archaeal, or bacterial-type flagellum-dependent cell motility | Flagellar motor rotation protein MotB | – | – | 15 | 14 |
| 4011 | 1.61 | Cell adhesion | Uncharacterized fimbrial-like protein ygiL precursor | ybgD | Ybg fimbriae | 15 | 12 |
| 3913 | 2.34 | Transport | Uncharacterized outer membrane usher protein yqiG precursor | ybgQ | Ybg fimbriae | 15 | 12 |
| 3991 | 1.98 | Pilus organization | Periplasmic fimbrial chaperone | ybgP | Ybg fimbriae | 15 | 12 |
| 3953 | 2.03 | Cell adhesion | FIG00637862: hypothetical protein | ybgO | Ybg fimbriae | 15 | 12 |
| 23 | 3.04 | Transposition, DNA-mediated | Mobile element protein | ybfL(partial) | DDE domain transposase family | 15 | 12 |
| 4242 | 4.23 | NA | orf2C hypothetical protein | ybfC | putative secreted protein | 15 | 9 |
| 68 | 8.49 | NA | core protein | rhsC | Rhs protein | 15 | 5 |
Differential expression is shown as log2 fold change. Identification and putative functions are based on gene ontology, annotation and blast against E. coli reference genomes and virulence factor databases.