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. 2018 Jun 29;9:1398. doi: 10.3389/fmicb.2018.01398

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Copyright © 2018 Sun, Lyu, Wen, Song, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction of OhrR with ohrR–ohrB2 intergenic region and ohrB1 promoter. (A) qRT-PCR of ohr expression in DohrR, CohrR and WT. RNA was prepared from cells grown in FM-I for 2 or 6 days. Values shown are mean ± SD from three technical replicates. ^∗∗P < 0.01; ^∗∗∗P < 0.001; NS, not significant (Student’s t-test). (B) EMSAs of His₆-OhrR with ohrR–ohrB2 intergenic region and ohrB1 promoter region. Competition assays were performed using 200-fold excess of specific (S) and non-specific (N) unlabeled DNAs. Top: concentrations of His₆-OhrR and DTT. Bottom: probes used. Arrow: free probe.