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. 2018 Jun 29;9:1398. doi: 10.3389/fmicb.2018.01398

FIGURE 2.

FIGURE 2

DNase I footprinting assay of ohrR–ohrB2 intergenic region using His6-OhrR. (A) Fluorograms corresponding to control DNA and to protected reactions with 0.4 and 0.8 μM His6-OhrR. (B) Nucleotide sequences of ohrR–ohrB2 intergenic region. Non-shaded boxes: presumed –35 and –10 regions of ohrR and ohrB2. Shaded boxes: regions protected by His6-OhrR. Underlining: OhrR motif (site a and site b). Gray bent arrows: translational start codons. Black bent arrows: TSSs. (C) Consensus sequence of OhrR motif.