Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Source |
|---|---|---|
| S. avermitilis | ||
| ATCC31267 | Wild-type strain (WT) | Laboratory stock |
| DohrR | ohrR deletion mutant | This study |
| CohrR | ohrR complementation strain | This study |
| E. coli | ||
| JM109 | General cloning host | Laboratory stock |
| BL21 (DE3) | Host for protein overexpression | Laboratory stock |
| Plasmids | ||
| pKC1139 | Multiple-copy, temperature-sensitive E. coli–Streptomyces shuttle vector | Bierman et al., 1992 |
| pSET152 | Integrative E. coli–Streptomyces shuttle vector | Bierman et al., 1992 |
| pET-28a (+) | Vector for His6-tagged protein overexpression in E. coli | Novagen |
| pKCD-ohrR | ohrR deletion vector based on pKC1139 | This study |
| pSET-ohrR | ohrR complementation vector based on pSET152 | This study |
| pET-OhrR | ohrR overexpression vector based on pET-28a (+) | This study |