Figure 2.

Alcohol-fed hepatocyte TLR4 deficient mice have significantly reduced mRNA expression of inflammatory genes in the epididymal fat pad (eWAT). A, mRNA expression of genes involved in inflammation in the liver (n = 3–5) of mice. B, mRNA expression of genes involved in inflammation in the eWAT (n = 3–4) of mice. Circulating concentrations of C, Tnfα (n = 6–11), D, Mcp1 (n = 5–9), and E, IL-1β (n = 5–9) in Tlr4^fl/fl and Tlr4^LKO mice after 4 weeks of alcohol-containing diet or pair-fed control diet. *p < 0.05, **p < 0.01, compared between mice of the same genotype fed different diets; ^#p < 0.05, compared between Tlr4^fl/fl and Tlr4^LKO mice on the same diet. Data are expressed as mean ± SEM.