Figure 4.

Significantly reduced TLR4 mRNA expression in peritoneal macrophages and stromal vascular fraction cells of alcohol-fed Tlr4^ΔmΦmice. Tlr4^fl/fl and Tlr4^ΔmΦ mice were fed either control or alcohol-containing diet for 4 weeks. (A) qPCR analysis of TLR4 mRNA expression in peritoneal macrophages (PM, n = 3–4). (B–C) qPCR analysis of TLR4 mRNA expression in stromal vascular fraction cells (SVF, B) and adipocytes (C) isolated from epididymal white adipose tissue (n = 3–4). *p < 0.05, ***p < 0.001, compared between mice of the same genotype fed different diets; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared between Tlr4^fl/fl and Tlr4^ΔmΦ mice on the same diet. All data are presented as mean ± SEM.