Figure 5.

Mice lacking myeloid cell TLR4 show significantly reduced circulating ALT levels after chronic alcohol intake. A, Plasma levels of ALT (n = 7–8). B, Plasma levels of AST (n = 7–8). C, Hepatic TBARS contents (n = 6–8). D, Liver triglyceride contents (n = 7–10). E, Hepatic mRNA expression of genes involved in pro-inflammation (n = 4–6). F, eWAT mRNA expression of genes involved in pro-inflammation (n = 5–6). Circulating concentrations of G, Tnfα (n = 6–11), H, Mcp1 (n = 6–11), and I, IL-1β (n = 6–11) in Tlr4^fl/fl and Tlr4^ΔmΦ mice after four weeks of alcohol-containing diet or pair-fed control diet. *p < 0.05, ***p < 0.001, compared between mice of the same genotype fed different diets; ^#p < 0.05, ^##p < 0.01, compared between Tlr4^fl/fl and Tlr4^ΔmΦ mice on the same diet. All data are presented as mean ± SEM.