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. 2018 Jun 5;14:108–120. doi: 10.1016/j.molmet.2018.05.019

figs4.

figs4

Secretagogin silencing reduces nuclear Pdx1 levels. (A–A1) shRNA-mediated Scgn silencing reduces nuclear Pdx1 immunoreactivity in INS-1E cells (EGFP+ cells; arrowheads in A1) relative to cells transfected with a control construct (EGFP+; arrowheads in A). Open arrowheads point to non-transfected cells with unchanged Pdx1 signal. Hoechst 33,342 was used as a nuclear counterstain (pseudo-coloured in grey). Scale bar = 5 μm. Quantitative analysis is shown in Figure 4C2.4