figs5.

Ca²⁺-dependent secretagogin-mediated control of gene expression with relevance to acquiring and maintaining β cell-like identity in INS-1E cells. (A–D) Secretagogin knock-down by shRNA (48 h) reduces, while secretagogin overexpression (CMV-Scgn; 48 h) up-regulates RNA expression of NeuroD1 (A), Pax4 (B), Nkx6.1 (C) and Pdx1 (D) in INS-1E cells. (E) Decreased Ins mRNA levels are seen upon transfection of INS-1E cells with shRNA targeting Scgn. In turn, transfection with CMV-Scgn significantly increases Ins RNA expression. Tbp mRNA was used as internal control. Data were expressed as means ± s.d. from triplicate experiments, ***p < 0.001, **p < 0.01, *p < 0.05 (one-way ANOVA).5