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. 2018 Jun 29;9:1419. doi: 10.3389/fmicb.2018.01419

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Copyright © 2018 Cao, Xu, Gao, Jiang, Liu, Fang, Gao, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Barley yellow striate mosaic virus (BYSMV) infection starts in the hindgut of SBPHs. Internal organs of infected SBPHs were immunolabeled for BYSMV with N-FITC (green) and stained for actin with phalloidin-rhodamine (red), then examined by confocal microscopy. (A) View of dissected alimentary canal of a negative control planthopper unexposed to BYSMV. At 1 (B), 2 (C,D), 3 (E), 5 (F), 7 (G–I), 11 (J), and 12 (K,L) days padp, internal organs of BYSMV-infected SBPHs were dissected, isolated, and processed for iCLSM. These images are representative of multiple experiments with multiple preparations. ad, anterior diverticulum; mg, midgut; hg, hindgut; mt, malpighian tubules; es, esophagus; sg, salivary gland; h, hemocytes; gl, gut lumen; me, midgut epithelium. Bar in hemocytes is equal to 10 μm. Other Bars, 100 μm.