Table 1.
Acquisition access period (AAP) and BYSMV-positive rate of SPBHs.
| AAP | Infectivity (No. of infected/total insects) |
|||
|---|---|---|---|---|
| Experiment 1 | Experiment 2 | Experiment 3 | Total (percentage) | |
| 1 h | 1/49 | 1/50 | 2/50 | 4/149(2.7%) |
| 4 h | 5/50 | 6/50 | 3/45 | 14/145(9.7%) |
| 24 h | 12/50 | 13/50 | 13/51 | 38/151(25.2%) |
| 36 h | 30/53 | 18/43 | 19/50 | 67/146(45.9%) |
| 48 h | 27/50 | 20/43 | 19/41 | 66/134(49.3%) |
Second-instar nymphs of SBPHs were fed on BYSMV-infected barley plants for different time acquisition access, then incubated on healthy barley seedling for 12 days. The whole alimentary canals were dissected for iCLSM test using BYSMV-N antibody conjugated with fluorescein isothiocyanate (N-FITC).