Table 2.

Statistics of BYSMV antigens in various organs/tissues of SBPHs.

Days padp	No. positive insects with N-FITC in different tissues
	Hindgut	Midgut	Esophagus	Anterior diverticulum	Salivary glands	Total number
2 days	17	0	0	0	0	45
3 days	21	2	0	0	0	50
5 days	24	10	4	2	0	46
7 days	24	23	17	10	7	50
12 days	26	26	26	26	25	47

Second-instar nymphs of SBPHs were fed on BYSMV-infected barley plants for a 36-h acquisition access, then incubated on healthy barley seedling for 2, 3, 5, 7, and 12-day padp. The whole internal organs were dissected for iCLSM test using BYSMV-N antibody conjugated with fluorescein isothiocyanate (N-FITC).