FIG 2 .

S. pneumoniae infection reduces MRP1 and increases MRP2 upon pulmonary infection in mice. Mice were infected via an intratracheal route with S. pneumoniae and sacrificed 2 days postinfection. The lungs were excised, reinflated, sectioned, and stained for MRP1, MRP2, MRP4, or MRP5. (A) Representative images from three different experiments. Arrows indicate points of interest. In particular, areas of similar density in MRP1-stained lungs appear to have reduced expression in infected lungs compared to uninfected lungs (arrow). MRP2 staining appears to increase drastically on the cell periphery during infection compared to mock-infected lungs (arrow). No such increases or decreases are observed for MRP4 and MRP5. (B) Quantification of staining. Antibody staining was quantified and normalized to surface area (measured by F-actin [not shown]). Fold differences of signal to surface area for each given antibody comparing infected (S. pneumoniae) to uninfected (Buffer) animals are shown. Values are expressed as fold increase or decrease compared to the values for uninfected samples.