FIG 3 .

MRP2 inhibition via probenecid reduces PMN migration across polarized pulmonary epithelial cells. (A and B) Polarized NCI-H292 cells on filter membranes were incubated with 100 µM MRP2 inhibitor probenecid or mock treated for 1 h with PBS before apical infection with S. pneumoniae at an MOI of 10 (A) or the HxA₃-independent bacterial product formyl-methionyl-leucyl-phenylalanine (fMLP) (B). For both panels A and B, mock-infected or mock-treated cells were exposed to HBSS for the treatment time period, and basolateral-to-apical migration of PMNs was quantified with a myeloperoxidase assay against a standard number of PMNs (see Materials and Methods). The results of one representative experiment from at least three experiments performed are shown. Statistical significance was calculated using two-tailed Student’s t test.