TABLE 1 .
Strains and plasmids used in this study
| Strain or plasmid | Strain genotype or plasmid construction | Source |
|---|---|---|
| Strains | ||
| CNVY106 | α H99::GFP-tubulin-NAT (pLKB35) | This study |
| CNVY108 | α H99::GFP-H4-NAT (pVY3) | 35 |
| CNVY111 | a KN99::mCherry-CENP-A-NEO (pLKB74) GFP-NDC1-NAT (pVY4) | 35 |
| CNVY138 | a KN99::mCherry-CENP-A-NEO (pLKB74) SAD1::SAD1-GFP-NAT | This study |
| CNVY156 | α H99::CENP-Ap-GFP-CENP-A-NAT (pVY22) | This study |
| CNVY177 | α SPC98p::GAL7p-SPC98-HYG SPC98::SPC98-GFP-NAT H99::mCherry-CENP-A-NEO (pLKB74) | This study |
| CNVY182 | α H99 SAD1p::GAL7p-mCherry-SAD1-HYG GFP-NDC1-NAT (pVY4) | This study |
| CNVY191 | α SAD1::sad1-NEO | This study |
| CNVY193 | a KN99::mCherry-CENP-A-HYG (pLKB75) GFP-NDC1-NAT (pVY4) SAD1::sad1-NEO | This study |
| CNVY194 | α H99::GFP-tubulin-NAT (pLKB35) SAD1::sad1-NEO | This study |
| CNVY200 | a KN99::CENP-Ap-GFP-CENP-A-NAT (pVY8) SAD1::sad1-NEO | This study |
| CNVY210 | α H99::GFP-H4-NAT (pVY3) SAD1::sad1-NEO | This study |
| Plasmids | ||
| pLKB35 | pCN19 + α-tubulin (BamHI-BamHI) | 35 |
| pLKB74 | pXLI + CENP-Ap-mCherry-CENP-A (NEO) | 35 |
| pLKB75 | pXLI + CENP-Ap-mCherry-CENP-A (HYG) | 35 |
| pVY1 | pCN19 + CENP-A (BamHI-BamHI) | 35 |
| pVY3 | pCN19 + H4 (BamHI-SpeI) | 35 |
| pVY4 | pCN19 + NDC1 (BamHI − SpeI) | 35 |
| pVY8 | GFP-CENP-A-NAT from pVY1 into pBSII KS using SacI-ApaI | This study |
| pVY22 | CENP-Ap replaced H3p in pVY8 | This study |