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. 2018 Jun 29;9:833. doi: 10.3389/fpls.2018.00833

FIGURE 4.

FIGURE 4

Genotyping and classifying ZmbZIP60 promoters. (A) 5000 bp upstream region of ZmbZIP60 showing location of primers for genotyping and classifying promoters as being B73 or CML type. Note CACTA-TE and 12 bp repeats in the B73 upstream region. (B) Genotyping the NAM founder and various inbred maize lines. Primer set 1 was used to determine the presence of the CACTA TE, primer set 2 was employed to identify motif 4 and primer set 3 was used for motif 5. Based on the presence or absence of these elements, all 32 maize lines could be categorized into one of three groups, B73 type (blue), CML247 type (red), and other type (green) The boxes outlined in yellow show B73 type motif 4 in lines NC350, NC358, and W22, however, they are located differently from the start of transcription than in B73. The designation “Other” indicates the promoter could not be classified by this criterion.