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A
Correlation analysis between final tumor mass and gastrocnemius muscle mass in the C26 inoculated cohorts from Table
1.
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B, C
BALB/C mice were intraperitoneally injected daily with either AICAR (500 mg/kg/day), metformin (250 mg/kg/day), or an equivalent volume of saline starting 12 days after C26 inoculation. Tissues were collected from mice either two days later (Day 14) or nine days later (Day 21). (B) Gastrocnemius muscle weights. (C) RT–qPCR analysis of Atrogin‐1/MAFbx and MuRF1 mRNA expression from the tibialis anterior at Day 14.
Data information: Results are derived from four mice per cohort for Day 14 (
n = 4) and three mice per cohort for Day 21 (
n = 3), and error bars represent the SEM. Significance between means was first determined using ANOVA. Significance
P‐values were calculated using Fisher's LSD. *
P < 0.05; **
P < 0.01 from saline controls or indicated comparisons.
