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. 2018 Jun 15;10(7):e8536. doi: 10.15252/emmm.201708536

Figure 2. PGE 2‐mediated MEF2 activation is Gi/o‐βγ dependent.

Figure 2

  • A
    Illustration of the target points of the used pharmacological and protein‐based inhibitors.
  • B–D
    (B, D) NRVMs were infected with the 3xMEF2‐Luciferase reporter and with recombinant adenoviruses encoding EGFP (AdGFP), RGS16, RGS2, p63ΔN, RGS‐LSCII, or Gαt, as indicated. The cells were serum starved for 20 h and stimulated with DMSO or 1 μM PGE2 for 24 h. (C) NRVMs were infected with the 3xMEF2‐Luciferase reporter and pretreated with the adenylyl cyclase inhibitor SQ22536 (100 μM) for 20 min or the Gi/o protein inhibitor pertussis toxin (PTX, 100 ng/ml) for 20 h in serum‐starved conditions and treated with DMSO or 1 μM PGE2 for 24 h.
Data information: In (B–D), n = 3, independent experiments, *represents significant interaction between the two treatments (P < 0.05, two‐way ANOVA, RGS16, P < 0.0001; RGS2, P = 0.1725; p63ΔN, P = 0.0666; RGS‐LSCII, P = 0.0818, SQ22536, P = 0.385; PTX, P < 0.0001; Gαt, P < 0.0001), values are mean ± s.e.m.Source data are available online for this figure.