Figure EV2. The PGE ₂‐induced MEF2 activity and protein synthesis is abolished by overexpression of the βγ‐scavenger RGS3L.

Left panel: NRVMs were infected on the first day with 3xMEF2‐Luc, on the next day with adenovirus encoding EGFP or RGS3L, then were serum starved. 24‐h vehicle or 1 μM PGE₂ stimuli were used. Overexpression RGS3L prevented the PGE₂‐induced response. Right panel: After infection with adenovirus encoding EGFP or RGS3L, NRVMs were starved, then treated with vehicle or 1 μM PGE₂ in media containing 1 μCi/ml [³H]‐leucine for 24 h. Thereafter, [³H]‐leucine uptake was assessed. RGS3L counteracted the effect of PGE₂. Values are mean + s.e.m., n = 3–4, technical replicates, two‐way ANOVA, *means significant interaction between RGS3L overexpression and the effect of PGE₂, P < 0.05 (MEF2 activity: P = 0.007, [³H]‐leucine uptake: P = 0.0393). The exact n and P‐values can also be found in the Source Data Excel file for Fig EV2.Source data are available online for this figure.