Figure 7. Vtn‐dependent B220⁺CD11c⁺NK1.1⁺HepEL attachment to fibrinogen.

• A
  Adhesion assay of B220⁺CD11c⁺NK1.1⁺ cells to fibrinogen‐coated plates. The rhodamine‐labelled liver B220⁺CD11c⁺NK1.1⁺ cells were separately cultured with TCM‐stimulating liver tissues and seeded on a fibrinogen‐coated plate.
• B
  Relative mRNA levels of FX, Vtn and TSP1 in liver B220⁺CD11c⁺NK1.1⁺ cells stimulated with LiCM or LuCM. Shown are averages (N = 6/group) with SEM and one‐way ANOVA.
• C
  Relative mRNA levels of FX in liver B220⁺CD11c⁺NK1.1⁺ cells stimulated with various factors. Shown are averages (N = 4) with SEM and one‐way ANOVA.
• D
  In vitro adhesion assay. Liver B220⁺CD11c⁺NK1.1⁺ cells were seeded on a fibrinogen plate. Cells attached to the plate were counted. Twenty μg/ml of anti‐Vtn Ab or isotype control IgG was used to block the adhesion. Shown are averages (N = 6/group) with SEM and one‐way ANOVA.
• E
  Lung B220⁺CD11c⁺NK1.1⁺ cells were seeded on ECM‐coated (fibrinogen, fibronectin or collagen I) plates. Shown are averages (N = 5/group) with SEM and one‐way ANOVA.