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. 2018 Jun 29;9:879. doi: 10.3389/fpls.2018.00879

FIGURE 5.

FIGURE 5

Functional analysis of Ma-GY62 IRT1, NRAMP1, ZIP4 and HMA3 expression in yeast strains. (A) Heterologous expression of genes MaIRT1, MaNRAMP1, MaZIP4, or MaHMA3 increased the Cd sensitivity of the ΔYcf1 strain. Growth of yeast strains transformed with the empty vector (pYPGE15) or MaIRT1, MaNRAMP1, MaZIP4 or MaHMA3 on synthetic defined medium with 0, 10, or 20 μM CdCl2 was assessed. (B) Expression of MaIRT1 or MaZIP4 increased the Zn sensitivity of the Δzrc strain. Growth of yeast strains transformed with the empty vector (pYPGE15) or with the genes MaIRT1, MaNRAMP1, MaZIP4, or MaHMA3 on synthetic defined medium with 0, 5, or 10 mM ZnSO4 was assessed. Yeast cells transformed with the empty vector or with genes MaIRT1, MaNRAMP1, MaZIP4, or MaHMA3 were grown in liquid SD medium containing (C) 5 μM CdCl2 or (D) 5 mM ZnSO4 for 30 h.