Table 1. Sequences and Abbreviations for Seven Synthetic Variants of the “M13” Peptidea.
| peptide | amino acid sequence |
|---|---|
| M13 | Ac-KRRWKKNFIAVSAANRFKKISSSGAL-NH2 |
| W4C*b | Ac-KRRC*KKNFIAVSAANRFKKISSSGALW-NH2 |
| F8C* | Ac-KRRWKKNC*IAVSAANRFKKISSSGAL-NH2 |
| S12C* | Ac-KRRWKKNFIAVC*AANRFKKISSSGAL-NH2 |
| R16C* | Ac-KRRWKKNFIAVSAANC*FKKISSSGAL-NH2 |
| I20C* | Ac-KRRWKKNFIAVSAANRFKKC*SSSGAL-NH2 |
| S23C* | Ac-KRRWKKNFIAVSAANRFKKISSC*GAL-NH2 |
a
Which is the CaM-binding domain of skeletal muscle myosin light chain kinase.
b
An addition of an additional tryptophan residue at the C-terminus was made to the W4C* peptide to facilitate the accurate determination of its concentration in solution.