Table 3. Line Shape Changes for the CN Stretching Bands (from Figure 2) of Probe-Labeled Peptides Bound to CaM^a.

peptide	Δmode (cm–1)	Δmean (cm–1)	Δfwhm (cm–1)	mean SASA (Å2)
W4C*	–8.6	–6.0	+3.1	0.25
F8C*	–5.8	–7.4	+1.1	0.01
S12C*	–6.7	–5.1	–0.8	6.33
R16C*	–1.9	–1.3	+4.4	6.46
I20C*	–2.9	–0.8	–1.5	7.92
S23C*	–1.0	–1.0	+0.7	17.6

^aFull line shape analysis parameters are reported in the Supporting Information. SASA values are calculated for the SCN group’s N atom.