Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 5;49:57. doi: 10.1186/s13567-018-0552-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Immunoblot analysis of optA, optB, LfliC and Lhly antigens expressed in JOL1800 derived strains, JOL1809, JOL1810, JOL1811 and JOL1812, respectively. The respective vaccine strains were grown in LB broth to mid-log phase and then the culture supernatants were collected and subjected to Western blot analysis using protein-specific hyperimmune sera raised in rabbits. The expression plasmid, pJHL65 transformed into JOL912 was used as a vector control. The predicted molecular mass of the expressed recombinant proteins were ~17 kDa for OptA, ~41.1 kDa for OptB, ~38.6 kDa for FliC and ~30 kDa for Hly. Lane M, size marker; lane C, vector control; lane OptA, a pellet of JOL1809; lane OptB, a pellet of JOL1810; lane FliC, a pellet of JOL1811; lane Hly, a pellet of JOL1812.