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. 2018 Jul 5;49:57. doi: 10.1186/s13567-018-0552-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mucosal immune response. Titers of intestinal secretory IgA (sIgA) (A) and virginal sIgA (B) obtained after vaccination with a co-mixture of all the four strains. The responses measured against each LI protein by an indirect ELISA using individual recombinant proteins as coating antigens. Each data points represent mean ± standard deviation (sd) of 10 mice/group. *P < 0.05. wpi: week post-immunization. ns: non-significant.