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. 2018 Jul 5;49:57. doi: 10.1186/s13567-018-0552-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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FACS analysis of splenic T lymphocytes. A Representative flow cytometry histogram plots for CD4⁺ and CD8⁺ splenic T cell populations. B Change in the T cell subpopulation in the control mice and immunized mice. Error bars indicate the sd. *P < 0.05 when the values were compared with those of non-immunized mice.