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. 2018 Jul 5;49:57. doi: 10.1186/s13567-018-0552-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cytokine assay in splenocytes following in vitro re-stimulation. The mRNA transcript levels of IL-4, IFN-γ and IL-17 were evaluated in the primed splenocytes pulsed in vitro with each antigen by performing RT PCR. The values of the relative fold change of each group were expressed as the mean ± SD. *P < 0.05 vs. the non-immunized control.