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. 2018 Jul 5;13:37. doi: 10.1186/s13020-018-0193-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Siegesbeckia pubescens Makino (SP) exhibited anti-inflammatory effects in Pam₃CSK₄-stimulated RAW 264.7 cells (n = 3). RAW 264.7 cells were pretreated with SP with different concentrations for 4 h before Pam₃CSK₄ (200 ng/mL) stimulation for another 12 h. a Nitric oxide (NO) was determined by Griess assay. TLR1/TLR2 antagonist: CU-CPT22 (CU) was selected as the positive control. We measured the levels of b IL-6, c TNF-α, and d MCP-1 by ELISA assay. *P < 0.05 vs. Pam₃CSK₄-induced, **P < 0.01 vs. Pam₃CSK₄-induced, ***P < 0.001 vs. Pam₃CSK₄-induced