Fig. 4.

let-7b regulates TSHR expression via targeting PLZF. The expression of PLZF and TSHR in thyroid tissue. Thyroid primary cells were isolated from fresh thyroid tissue and cultured in vitro. Negative control or let-7b mimics, Inhibitor negative control, or let-7b inhibitors were transfected into primary thyroid cells (a, c) and human normal thyroid primary cells (b, d) and PLZF mRNA (a, b) and TSHR mRNA (c, d) was detected by real-time PCR. Results were calculated by three independent experiments, *P < 0.05, **P < 0.001