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. 2018 Jul 5;14:47. doi: 10.1186/s13223-018-0270-9

Table 2.

Primer sequences used in real-time PCR

Gene Size (bp) Forward and reverse primers
T-bet 115 F: 5′- CTGGAGGTGTCGGGGAAAC-3′
R: 5′-ATGGGAACATCCGCCGTCC-3′
GATA-3 107 F: 5′-TCATTAAGCCCAAGCGAAGG-3′
R: 5′-GTCCCCATTGGCATTCCTC-3′
Ror-γt 144 F: 5′- AGACTCATCGCCAAAGCA -3′
R: 5′- CCTTGTAGAGTGGAGGGAAA -3′
FoxP-3 124 F: 5′-ATTCCCAGAGTTCCTCCACAAC-3′
R: 5′-ATTGAGTGTCCGCTGCTTCTC-3′
β-Actin 131 F: 5′-TCCCTGGAGAAGAGCTACG-3′
R: 5′-GTAGTTTCGTGGATGCCACA-3′