Fig. 4.

PS was infused into the bladder and 24 hours later, there was a significant increase in the permeability of the colon shown as the decrease in TEER (A) and increase in G (B) in vehicle-treated rats (n = 7/13) compared with sham controls (n = 7/14). Linaclotide (n = 8/15) reversed the PS-induced changes in colonic permeability as assessed via TEER (A) and G (B) (n = X/X denotes the number of animals/number of chambers). Linaclotide had no significant effect on PS-induced changes in urinary bladder permeability, as assessed via TEER (C) and G (D) (n = 8). Data are expressed as the mean ± S.E.M. Statistical significance was assessed by one-way ANOVA followed by the Tukey post hoc test for multiple comparisons ***P < 0.001; ****P < 0.0001 (compared with sham plus vehicle); ^†P < 0.005; ^†††P < 0.001 (compared with PS plus vehicle). LIN, linaclotide; VEH, vehicle.